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| 产品名称： | CCD-1074Sk |
|---|---|
| 商品货号： | TS212465 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 42 years |
| Gender： | female |
| Applications： | The line was established from skin taken from normal breast tissue removed at mastectomy. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established from skin taken from normal breast tissue removed at mastectomy. |
| Clinical Data： | female |
| Comments： | The line was established from skin taken from normal breast tissue removed at mastectomy. Cells senesce after approximately 26 population doublings. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |