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微信小章| 产品名称： | CCD 1106 KERTr |
|---|---|
| 商品货号： | TS212468 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | keratinocyte; human papillomavirus 16 (HPV-16) E6/E7 transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Biosafety Level： | 2 Cells may contain the Human Papilloma viral (HPV) sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 133 days gestation |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Passage 2 cells were transformed with a retrovirus vector (LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene. |
| Antigen Expression： | epithelial specific antigen |
| Oncogene： | E6/E7 + |
| Genes Expressed： | E6/E7 +, epithelial specific antigen |
| Comments： | This line stains positively with antibody for cytokeratin. After 50 population doublings, the cells continue dividing and retain cuboidal morphology. E6E7 sequences were detected by PCR in cells at passage 18. Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.
Epithelial specific antigen was detected using antibody produced by the Ep16 hybridoma (ATCC HB-155). |
| Complete Growth Medium： | These cells are grown in Keratinocyte-Serum Free Medium (Gibco 17005-042) with added Keratinocytes Supplements (Gibco 37000-015) including Bovine Pituitary Extract (BPE; Gibco 13028-014) and human recombinant epidermal growth factor (EGF; Gibco 10450-013) further supplemented with:xa0
Do not filter the complete medium. xa0 This medium is formulated for use with a 5% CO2 in air atmosphere. |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:5 Medium Renewal: Twice a week.
Note:xa0Add fresh medium twice per week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Freeze Medium: Hams F12 medium 85%; DMSO, 5%; FBS 10%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | L Vilner |
| Passage History： | E6E7 sequences were detected by PCR in cells at passage 18. Passage 2 cells were transformed with a retrovirus vector ( LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene. |
| References： | Zabrenetzky V, et al. The isolation, immortalization and characterization of human fetal keratinocytes. In Vitro Cell. Dev. Biol. 33: Part II, p. 34A, 1997. |