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微信小章| 产品名称： | CC63 |
|---|---|
| 商品货号： | TS212472 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for bovine CD8 T lymphocyte antigen. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with NS1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against bovine CD8 (BoCD8) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against bovine CD8 (BoCD8) |
| Comments： | Animals were immunized with bovine thymocytes. Spleen cells were fused with NS1 myeloma cells. The antibody is specific for bovine CD8 T lymphocyte antigen. This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium.
Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | mouse IgG2a |
| Name of Depositor： | CJ Howard |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Seidler RJ, et al. L-selectin expression differentiates T cells isolated from different lymphoid tissues in cattle but does not correlate with memory. Immunology 77: 228-234, 1992. PubMed: 1385313 Howard CJ, et al. Immunity to bovine virus diarrhoea virus in calves: the role of different T-cell subpopulations analysed by specific depletion in vivo with monoclonal antibodies. Vet. Immunol. Immunopathol. 32: 303-314, 1992. PubMed: 1321529 Howard CJ, et al. Distinction of naive and memory BoCD4 lymphocytes in calves with a monoclonal antibody, CC76, to a restricted determinant of the bovine leukocyte-common antigen, CD45. Eur. J. Immunol. 21: 2219-2226, 1991. PubMed: 1716214 Oldham G, et al. In vivo role of lymphocyte subpopulations in the control of virus excretion and mucosal antibody responses of cattle infected with rotavirus. J. Virol. 67: 5012-5019, 1993. PubMed: 8392626 |