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微信小章| 产品名称： | CC8 |
|---|---|
| 商品货号： | TS212473 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for the 50000 dalton bovine CD4 molecule. |
| Derivation： | Spleen cells were fused with NS1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against bovine CD4 (BoCD4) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against bovine CD4 (BoCD4) |
| Comments： | Animals were immunized with bovine lymphocytes. Spleen cells were fused with NS1 myeloma cells. The antibody is specific for the 50000 dalton bovine CD4 molecule. The CD4 antigen is expressed on T cells that are CD2 positive and bear the alpha/beta T cell receptor. This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989. |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG2a |
| Name of Depositor： | CJ Howard |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Howard CJ, et al. In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764, 1989. PubMed: 2567247 Seidler RJ, et al. L-selectin expression differentiates T cells isolated from different lymphoid tissues in cattle but does not correlate with memory. Immunology 77: 228-234, 1992. PubMed: 1385313 Howard CJ, et al. Two monoclonal antibodies (CC17, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5. Vet. Immunol. Immunopathol. 19: 127-139, 1988. PubMed: 3055661 Oldham G, Howard CJ. Suppression of bovine lymphocyte responses to mitogens following in vivo and in vitro treatment with dexamethasone. Vet. Immunol. Immunopathol. 30: 161-177, 1992. PubMed: 1534431 Howard CJ, et al. Immunity to bovine virus diarrhoea virus in calves: the role of different T-cell subpopulations analysed by specific depletion in vivo with monoclonal antibodies. Vet. Immunol. Immunopathol. 32: 303-314, 1992. PubMed: 1321529 Howard CJ, et al. Distinction of naive and memory BoCD4 lymphocytes in calves with a monoclonal antibody, CC76, to a restricted determinant of the bovine leukocyte-common antigen, CD45. Eur. J. Immunol. 21: 2219-2226, 1991. PubMed: 1716214 |