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微信小章| 产品名称： | CCD 1105 KIDTr |
|---|---|
| 商品货号： | TS212474 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney |
| Cell Type： | human papillomavirus 16 (HPV-16) E6/E7 transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Human Papilloma (HPV16) sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 133 days gestation |
| Applications： | Passage 3 cells were transformed with a retrovirus vector (LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene. The transformed cells are resistant to G418 and sensitive to Hygromycin B. E6E7 sequences were detected by PCR in cells at passage 15. Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens. CCD 1105 KIDTr is sensitive to Coxsackieviruses A9 and B5, measles virus, poliovirus 1 and rhinovirus. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Passage 3 cells were transformed with a retrovirus vector (LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene.
E6E7 sequences were detected by PCR in cells at passage 15. |
| Oncogene： | E6/E7 + |
| Genes Expressed： | keratin; acid phosphatase,E6/E7 + |
| Cellular Products： | keratin; acid phosphatase |
| Comments： | Fetal kidney was digested with collagenase: trypsin mixture. The digestion products were plated in REGM (Renal Epithelial Cell Growth Medium which include 0.5% FBS) from Clonetics on collagen l-fibronectin-BSA coated flasks. Passage 3 cells were transformed with a retrovirus vector (LXSN16E6E7 produced by ATCC CRL-2203) in the presence of polybrene. The transformed cells are resistant to G418 and sensitive to Hygromycin B. This line stains positively with antibody for cytokeratin and is acid phosphatase positive. After 50 population doublings, the cells continue dividing and retain cuboidal morphology. E6E7 sequences were detected by PCR in cells at passage 15. Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens. CCD 1105 KIDTr is sensitive to Coxsackieviruses A9 and B5, measles virus, poliovirus 1 and rhinovirus. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:5 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | V Zabrenetzky, A Thompson |
| References： | Zabrenetzky V, et al. The isolation, immortalization and characterization of human fetal keratinocytes. In Vitro Cell. Dev. Biol. 33: Part II, p. 34A, 1997. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |