微信小陈
微信小章
产品简介
购买须知
| 产品名称： | CC51 |
|---|---|
| 商品货号： | TS212478 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for a 145000 dalton molecule that is the bovine analogue of human CD21. |
| Derivation： | Spleen cells were fused with NS1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against bovine CD21 (BoCD21) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against bovine CD21 (BoCD21) |
| Comments： | Animals were immunized with mesenteric lymph node cells from a Friesian cow. Spleen cells were fused with NS1 myeloma cells. The antibody is specific for a 145000 dalton molecule that is the bovine analogue of human CD21. The antigen is expressed on bovine B cells and not on bovine T cells. |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG2b |
| Name of Depositor： | CJ Howard |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Naessens J, et al. Characterization of a bovine leucocyte differentiation antigen of 145,000 MW restricted to B lymphocytes. Immunology 69: 525-530, 1990. PubMed: 2185984 |