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| 产品名称： | CC56 |
|---|---|
| 商品货号： | TS212486 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with bovine B cells in peripheral blood and lymph nodes. |
| Derivation： | Spleen cells were fused with NS1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against bovine B cell |
| Cellular Products： | immunoglobulin; monoclonal antibody; against bovine B cell |
| Comments： | Animals were immunized with bovine mesenteric lymph node cells. Spleen cells were fused with NS1 myeloma cells. The antibody reacts with bovine B cells in peripheral blood and lymph nodes. It precipitates a 180000 dalton molecule from the surface of bovine B cells. This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992. |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG2a |
| Name of Depositor： | CJ Howard |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Hein WR, et al. Epitopes of the T19 lymphocyte surface antigen are extensively conserved in ruminants. Vet. Immunol. Immunopathol. 27: 173-181, 1991. PubMed: 1708546 . . Vet. Immunol. Immunopathol. 32: 1993.. |