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微信小章| 产品名称： | Capan-1 |
|---|---|
| 商品货号： | TS212493 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas; derived from metastatic site: liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 40 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Karyotype： | (P7) hypotriploid with abnormalities including dicentrics, breaks, acrocentric fragments, large submetacentric and subtelocentric chromosomes plus minute marker |
| Images： |  |
| Clinical Data： | male 40 years Caucasian |
| Antigen Expression： | Blood Type A; Rh+; HLA A2, A9, B13, B17 |
| Receptor Expression： | Progesterone receptor, positiveRef  Hollande E, et al. Expression of estrogen receptors during growth of human pancreatic adenocarcinoma cells (Capan-1)-relationship with differentiation. In Vitro Cell. Dev. Biol. Anim. 34: 593-599, 1998. PubMed: 9719420,xa0estrogen receptor, alpha, positivexa0RefHollande E, et al. Expression of estrogen receptors during growth of human pancreatic adenocarcinoma cells (Capan-1)-relationship with differentiation. In Vitro Cell. Dev. Biol. Anim. 34: 593-599, 1998. PubMed: 9719420 |
| Genes Expressed： | mucin, Blood Type A; Rh+; HLA A2, A9, B13, B17 |
| Cellular Products： | mucin |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms adenocarcinoma consistent with pancreatic duct carcinoma |
| Comments： | The cells will slough off of the growth surface if they become too heavy. Capan-1 expresses the cystic fibrosis transmembrane conductance regulator (CFTR) and secrete gastric type mucins. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： |
Medium Renewal:xa0Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 9 D16S539: 13,14 D5S818: 11 D7S820: 10,11 THO1: 6 TPOX: 8,11 vWA: 16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | J Fogh |
| Deposited As： | Homo sapiens |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Fanjul M, Hollande E. Morphogenesis of "duct-like" structures in three-dimensional cultures of human cancerous pancreatic duct cells (Capan-1). In Vitro Cell. Dev. Biol. Anim. 29A: 574-584, 1993. PubMed: 8354666 Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 Chambers JA, Harris A. Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells. J. Cell Sci. 105: 417-422, 1993. PubMed: 7691840 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Hollande E, et al. Expression of estrogen receptors during growth of human pancreatic adenocarcinoma cells (Capan-1)-relationship with differentiation. In Vitro Cell. Dev. Biol. Anim. 34: 593-599, 1998. PubMed: 9719420 |