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| 产品名称： | Capan-2 |
|---|---|
| 商品货号： | TS212494 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas |
| Product Format： | frozen |
| Morphology： | polygonal |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 56 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 69; range = 65 to 74. This is a pseudotriploid human cell line with the modal chromosome number of 69, occurring in 24% of cells. However, cells having chromosome counts from 65 to 70 also occurred at high frequencies. The rate of cells with higher ploidies was 7%. There were eight marker chromosomes common to most cells. They included paired del(1) (p22), t(8q11q), i(14q), single i(7p) and four others. Two more markers were seen only in some cells. Only one normal X chromosome was found in G-banded preparation. The Y chromosome was not detected in the Q-banded preparation. |
| Images： |  |
| Clinical Data： | 56 years Caucasian male |
| Antigen Expression： | Antigen expression: Blood Type B; Rh+ |
| Genes Expressed： | Blood Type B; Rh+,xa0 The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene. |
| Cellular Products： | mucin (apomucin, MUC-1, MUC-2) |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms well differentiated adenocarcinoma consistent with pancreatic carcinoma |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 11,12 D16S539: 9,13 D5S818: 11,12 D7S820: 9,11 THO1: 9.3 TPOX: 8 vWA: 17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 2 |
| Name of Depositor： | J Fogh |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1975 |
| References： | Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822 |