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| 产品名称： | CAL 27 |
|---|---|
| 商品货号： | TS212500 |
| Organism： | Homo sapiens, human |
| Tissue： | tongue |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma |
| Age： | 56 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | aneuploid; modal number = 43 |
| Derivation： | Cal 27 was established in 1982 by J. Gioanni (Centre Antoine Lacassagne, Nice Cedex, France) from tissue taken prior to treatment from a 56 year old Caucasian male with a lesion of the middle of the tongue. |
| Clinical Data： | 56 years
Caucasian
male |
| Tumorigenic： | Yes |
| Effects： | Yes, solid tumors developed within 6 weeks in nude mice inoculated with 2 x 106 cells subcutaneously |
| Comments： | CAL 27 cells are epithelial, polygonal with a highly granular cytoplasm.
Immunocytochemical studies show strong positive staining with anti keratin antibodies.
The cells do not grow well in semi-solid medium.
Marked inhibition of thymidine incorporation was observed in the presence of VP16 (etoposide), CCNU (1-2-chloroethyl-3-cyclohexyl-1-nitrosourea), VM26 (teniposide), ADM (adriamycin), CPA (cyclophosphamide), and MTX (methotrexate).
CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D).
A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 10,11 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 6,9.3 TPOX: 8 vWA: 14,17 |
| Population Doubling Time： | 35 hrs |
| Name of Depositor： | C Cardona |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1982 |
| References： | Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269 |