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| 产品名称： | CAMA-1 |
|---|---|
| 商品货号： | TS212504 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland/breast; derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | Adherent patches of epithelial cells; compact, multilayered colonies, rarely become confluent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 51 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 80; range = 68 to 83. This is a hypertriploid human cell line with the modal chromosome number of 80 occurring in 44% of a total of 88 cells examined. The rate of polyploid cells was 3.5%. Karyotypes of this cell line were generally uniform and stable. There were 12-13 marker chromosomes per cell, 11 of which were found in all cells, and 7 of which were mostly paired. Among the markers were paired mar(1qter--q21::?::6p11.2--6pter), t(3q::?), and i(16q); single der(12)t(12;?)(q24:?); and 8-9 others. Double minutes occurred in some cells; however, they were present as only one or two copies per cell. Structurally normal N16 was absent and N8 occurred in only a few cells. Paired normal X chromosomes were present in every cell. |
| Derivation： | The CAMA-1 line was established by J. Fogh at Sloan-Kettering in 1975 from cells in the pleural effusion of a patient with carcinoma of the breast. |
| Clinical Data： | 51 years
Caucasian
female |
| Antigen Expression： | Blood type O; Rh +; HLA A10, A11, B12, B18 |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice |
| Comments： | An ampule frozen in May of 1978 at passage 21 was provided to the ATCC in June of 1991.
The cells form poorly differentiated grade III tumors consistent with breast carcinoma. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 12 D16S539: 11 D5S818: 12,13 D7S820: 8,11 THO1: 8,9.3 TPOX: 8 vWA: 15 |
| Isoenzymes： | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor： | J Fogh |
| Deposited As： | Homo sapiens |
| Passage History： | An ampule frozen in May of 1978 at passage 21 was provided to the ATCC in June of 1991. |
| Year of Origin： | 1975 |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 |