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微信小章| 产品名称： | Caki-1 |
|---|---|
| 商品货号： | TS212505 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney; Derived from metastatic site: skin |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | clear cell carcinoma |
| Age： | 49 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 68; range = 63 to 71. The cell line is aneuploid human, with chromosome counts in the triploid range. The Y chromosome is absent; however, loss of the Y chromosome is not unusual from male tumor cell lines. All normal autosomes except chromosome N9 and N19 are represented, usually by two or three copies. Chromosome N9 is recognized as a marker chromosome (M1) that is usually trisomic. Normal chromosome N5 is and chromosomes N10 and N16 tend to be over-represented with respect to the copy number of other normal chromosomes. Thirteen marker chromosomes are identified: 9q+, t(1p;?), t(1qter>1q21::20), t(1q17q), der(11)t(3;11)(q21;q14), 19q+, 4q+, 4p+ and others. The chromosome counts and general cytogenetic features are in keeping with those described by J. Fogh, et al., J. Natl. Cancer Inst. (Bethesda) 58: 209, 1977. |
| Images： |  |
| Derivation： | Caki-1 was derived from a human tumor. |
| Clinical Data： | 49 years Caucasian male |
| Antigen Expression： | Blood Type O; Rh-; HLA A9, B12, Bw35 |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms clear cell carcinoma in nude mice consistent with renal primary; also forms tumors in steroid treated hamsters |
| Comments： | Ultrastructural features include many microvilli, few filaments, many small mitochondria, well developed Golgi and ER, many lipid droplets and multilaminate bodies, secondary lysosomes, no virus particles. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 11,12 D7S820: 8,12 THO1: 6,8 TPOX: 8,11 vWA: 15,17 |
| Isoenzymes： | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | J Fogh |
| Deposited As： | Homo sapiens |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 |