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| 产品名称： | Caov-4 |
|---|---|
| 商品货号： | TS212506 |
| Organism： | Homo sapiens, human |
| Tissue： | ovary; derived from metastatic site: subserosa of fallopian tube |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 45 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Karyotype： | (P5) hypotriploid and hypertetraploid with abnormalities including breaks, dicentrics, minute chromatin bodies, fragmentations, secondary constrictions and large subtelocentric and submetacentric markers |
| Images： |  |
| Clinical Data： | 45 years Caucasian female |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes).Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days Subcultivation Ratio:xa0A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal:xa02 to 3 times per week |
| Cryopreservation： | Freeze medium: complete growth medium supplemented with an additional 40% fetal bovine serum and 10% (v/v) DMSO |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 100% |
| Isoenzymes： | AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1-2 PGM3, 2 |
| Name of Depositor： | J Fogh |
| Deposited As： | Homo sapiens |
| References： | Yaginuma Y, et al. Analysis of the Rb gene and cyclin-dependent kinase 4 inhibitor genes (p16INK4 and p15INK4B) in human ovarian carcinoma cell lines. Exp. Cell Res. 233: 233-239, 1997. PubMed: 9194486 |