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微信小章| 产品名称： | C7 |
|---|---|
| 商品货号： | TS212509 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with bovine and human LDL receptor, and can be used to affinity purify LDL receptors. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Mice were immunized with partially purified bovine adrenal LDL receptor. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Antigen Expression： | H-2d |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor,H-2d |
| Cellular Products： | immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor |
| Comments： | Mice were immunized with partially purified bovine adrenal LDL receptor. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with bovine and human LDL receptor, and can be used to affinity purify LDL receptors. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 viable cells/mL. Maintain cell density between 5 x 104 and 5 x 105 viable cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG2b |
| Name of Depositor： | JL Goldstein |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Beisiegel U, et al. Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J. Biol. Chem. 256: 11923-11931, 1981. PubMed: 6271765 Schneider WJ, et al. Purification of the low density lipoprotein receptor, an acidic glycoprotein of 164,000 molecular weight. J. Biol. Chem. 257: 2664-2673, 1982. PubMed: 6277909 Beisiegel U, et al. Immunoblot analysis of low density lipoprotein receptors in fibroblasts from subjects with familial hypercholesterolemia. J. Biol. Chem. 257: 13150-13156, 1982. PubMed: 6290495 Tolleshaug H, et al. Post translational processing of the LDL receptor and its genetic disruption in familial hypercholesterolemia. Cell 30: 715-724, 1982. PubMed: 6291781 Tolleshaug H, et al. The LDL receptor locus in familial hypercholesterolemia: multiple mutations disrupt transport and processing of a membrane receptor. Cell 32: 941-951, 1983. PubMed: 6299582 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |