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| 产品名称： | CA3-F4 |
|---|---|
| 商品货号： | TS212515 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Lewis a (human blood group antigen) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Lewis a (human blood group antigen) |
| Comments： | Mice were immunized with naked Salmonella minnesota to which had been adsorbed neutral glycolipids from human Type A meconium. Spleen cells were fused with NS-1 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | WW Young |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Young WW Jr., et al. Characterization of monoclonal antibodies specific for the Lewis a human blood group determinant. J. Biol. Chem. 258: 4890-4894, 1983. PubMed: 6833281 Galanos C, et al. Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides. Eur. J. Biochem. 24: 116-122, 1971. PubMed: 4944989 |