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微信小章| 产品名称： | C-4 II |
|---|---|
| 商品货号： | TS212519 |
| Organism： | Homo sapiens, human |
| Tissue： | cervix |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain human papilloma virus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | 41 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This is one of two lines developed from one patient (see ATCC CRL-1594). |
| Clinical Data： | female
Caucasian
41 years |
| Comments： | The line contains human papillomavirus type 18 (HPV-18) DNA sequences, and expresses HPV-18 RNA. |
| Complete Growth Medium： | Waymouths MB 752/1 medium, 90%; fetal bovine serum, 10%
|
| Subculturing： | Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 3 times per week |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 11,12 D16S539: 11 D5S818: 9,11 D7S820: 10,11 THO1: 9,9.3 TPOX: 10,11 vWA: 14 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | N Auersperg |
| Deposited As： | Homo sapiens |
| References： | Auersperg N, Hawryluk AP. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix. J. Natl. Cancer Inst. 28: 605-627, 1962. PubMed: 13863218 Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 Auersperg N. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines. J. Natl. Cancer Inst. 43: 151-173, 1969. PubMed: 5796380 Auersperg N. Histogenetic behavior of tumors. II. Roles of cellular and environmental factors in the in vitro growth of carcinoma cells. J. Natl. Cancer Inst. 43: 175-190, 1969. PubMed: 5796382 Auersperg N. Histogenetic behavior of tumors. 3. Possible relationships to patterns of glycolysis. J. Natl. Cancer Inst. 48: 1589-1596, 1972. PubMed: 5056250 Lee HC, Auersperg N. Calcium in epithelial cell contraction. J. Cell Biol. 85: 325-336, 1980. PubMed: 6768754 |