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微信小章| 产品名称： | C-4 I |
|---|---|
| 商品货号： | TS212520 |
| Organism： | Homo sapiens, human |
| Tissue： | cervix |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain human papilloma virus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | 41 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This is one of two lines developed from this patient (see ATCC CRL 1595). |
| Clinical Data： | This is one of two lines developed from this patient (see ATCC CRL-1595).
female
Caucasian
41 years |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | This is one of two lines developed from this patient (see ATCC CRL-1595).
The line contains human papillomavirus type 18 (HPV-18) DNA sequences, and expresses HPV-18 RNA.xa0ATCC confirmed this cell line is positive for the presence of HPV viral DNA sequences via PCR.
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| Complete Growth Medium： | Waymouths MB 752/1 medium, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 3 times per week
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| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 11,12 D16S539: 11 D5S818: 9,11 D7S820: 10,11 THO1: 9.3 TPOX: 10,11 vWA: 14 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | N Auersperg |
| Deposited As： | Homo sapiens |
| References： | Auersperg N, Hawryluk AP. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix. J. Natl. Cancer Inst. 28: 605-627, 1962. PubMed: 13863218 Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Herz F, et al. Chromosome analysis and alkaline phosphatase of C41, a cell line of human cervical origin distinct from HeLa. Cancer Res. 37: 3209-3213, 1977. PubMed: 560251 Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 Auersperg N. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines. J. Natl. Cancer Inst. 43: 151-173, 1969. PubMed: 5796380 Auersperg N. Histogenetic behavior of tumors. II. Roles of cellular and environmental factors in the in vitro growth of carcinoma cells. J. Natl. Cancer Inst. 43: 175-190, 1969. PubMed: 5796382 Auersperg N. Histogenetic behavior of tumors. 3. Possible relationships to patterns of glycolysis. J. Natl. Cancer Inst. 48: 1589-1596, 1972. PubMed: 5056250 |