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微信小章| 产品名称： | C3H/MCA clone 15 |
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| 商品货号： | TS212521 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Strain： | C3H |
| Applications： | The line was derived by treating C3H/10T1/2 clone 8 cells with 3-methylcholanthrene. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | The line was derived by treating C3H/10T1/2 clone 8 cells with 3-methylcholanthrene. |
| Antigen Expression： | H-2k |
| Genes Expressed： | H-2k |
| Tumorigenic： | Yes |
| Effects： | Yes, in C3H mice |
| Comments： | The line was derived by treating C3H/10T1/2 clone 8 cells with 3-methylcholanthrene. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is Eagles Basal medium with 2 mM L-glutamine , 1.5 g/L sodium bicarbonate and Earles BSS. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: 2 to 3 times per week Cultures are prepared using 0.1% trypsin in calcium and magnesium free phosphate buffered saline. New cultures are seeded at 3 X 10 exp3 viable cells/sq cm. |
| Name of Depositor： | C Heidelberger |
| Deposited As： | Mus musculus |
| References： | Reznikoff CA, et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800 Terzaghi M, Little JB. Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940 Mondal S, Heidelberger C. Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242 Rapp UR, et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619 Chan GL, Little JB. Induction of oncogenic transformation in vitro by ultraviolet light. Nature 264: 442-444, 1976. PubMed: 1004574 Reznikoff CA, et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355 |