微信小陈
微信小章| 产品名称： | C58(NT)D.1.G.OUAR.1 |
|---|---|
| 商品货号： | TS212525 |
| Organism： | Rattus norvegicus, rat |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | WFu |
| Applications： | The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain. The line may be useful for the production of rat - rat T cell hybridomas. |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Comments： | The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain. The line may be useful for the production of rat - rat T cell hybridomas. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
|
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | R Hyman |
| Deposited As： | Rattus sp. |
| References： | Hyman R, Trowbridge I. Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants. Immunogenetics 12: 511-523, 1981. PubMed: 7194313 Geering G, et al. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J. Exp. Med. 124: 753-772, 1966. PubMed: 4288480 Curling E, et al. Isolation of rat/rat T cell hybrids using W/Fu C58(NT)D as fusion partner. J. Immunol. Methods 108: 171-178, 1988. PubMed: 2965188 |