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| 产品名称： | C32 |
|---|---|
| 商品货号： | TS212530 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | melanoma, amelanotic |
| Age： | 53 year old adult |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | C32 was derived from a human male with skin melanoma. |
| Clinical Data： | 53 year old adult Caucasian male |
| Tumorigenic： | Yes |
| Effects： | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12 D16S539: 11,12 D5S818: 12 D7S820: 9,11 THO1: 9.3 TPOX: 9 vWA: 14,19 |
| Name of Depositor： | TR Chen |
| Deposited As： | Homo sapiens |
| References： | Chen TR. Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277-284, 1978. PubMed: 277716 Chen TR, Shaw MW. Stable chromosome changes in human malignant melanoma. Cancer Res. 33: 2042-2047, 1973. PubMed: 4725368 |