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微信小章| 产品名称： | C3H/10T1/2, Clone 8 |
|---|---|
| 商品货号： | TS212536 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | sarcoma |
| Age： | embryo |
| Strain： | C3H |
| Applications： | This line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Mouse karyotype with a modal number of 80 chromosomes. |
| Images： |  |
| Derivation： | C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells. |
| Clinical Data： | The depositor recommends that the line be used between the 5th and 15th passages only. |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Ref   Smith GJ, et al. Clonal analysis of the expression of multiple transformation phenotypes and tumorigenicity by morphologically transformed 10T1/2 cells. Cancer Res. 53: 500-508, 1993. PubMed: 8425183RefReznikoff CA, et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355Yes, in semisolid medium Ref Smith GJ, et al. Clonal analysis of the expression of multiple transformation phenotypes and tumorigenicity by morphologically transformed 10T1/2 cells. Cancer Res. 53: 500-508, 1993. PubMed: 8425183RefReznikoff CA, et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355 |
| Comments： | The cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses.
The cells are contact sensitive.
There is no detectable background spontaneous transformation.
They are highly susceptible to transformation by chemical agents.
Tested and found negative for ectromelia virus (mousepox).
Note: the inoculation density, feeding and harvesting schedules must be followed rigidly if the line is to retain its essential characteristics.
The batch of serum used for growth and for transformation assays may affect both the morphology of this line and the results obtained.
Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks.
The depositor recommends that the line be used between the 5th and 15th passages only. |
| Complete Growth Medium： | The base medium for this cell line is Eagles Basal medium (Thermofisher Catalog No. 21010-046) supplemented with heat-inactivated fetal bovine serum to a final concentration of 10% and 2mM L-glutamine (ATCC 30-2214). |
| Subculturing： | Never allow the culture to become completely confluent.xa0 Subcultivation of cultures must be performed before they reach confluence. xa0Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: Seed new flasks at 2000 viable cells/cm2.
Medium Renewal: Once between subcultures if necessary |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid notrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | C Heidelberger |
| Deposited As： | Mus musculus |
| Passage History： | The donor recommends that the line be used between the 5th and 15th passages only. |
| References： | Reznikoff CA, et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800 Terzaghi M, Little JB. Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940 Mondal S, Heidelberger C. Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242 Smith GJ, et al. Clonal analysis of the expression of multiple transformation phenotypes and tumorigenicity by morphologically transformed 10T1/2 cells. Cancer Res. 53: 500-508, 1993. PubMed: 8425183 Rapp UR, et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619 Reznikoff CA, et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355 Jain MK, et al. Molecular cloning and characterization of SmLIM, a developmentally regulated LIM protein preferentially expressed in aortic smooth muscle cells. J. Biol. Chem. 271: 10194-10199, 1996. PubMed: 8626582 |