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| 产品名称： | C13589 |
|---|---|
| 商品货号： | TS212539 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transforme |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 Cells contain EBV viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | fragile X |
| Age： | 39 years adult |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line can be used a standard for sizing. |
| Storage Conditions： | -135°C |
| Clinical Data： | female Caucasian 39 years adult |
| Comments： | This cell line can be used a standard for sizing. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
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| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml. Maintain cell density between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: -135°C |
| Culture Conditions： | Temperature: 37.0°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 8,12 D16S539: 9,10 D5S818: 12,13 D7S820: 9 THO1: 6,8 TPOX: 10,11 vWA: 15 |
| Name of Depositor： | SL Nolin |
| Deposited As： | human |
| References： | The line was established from mononuclear cells taken from a fragile-X premutation female with 31 and 59 CGG-triplet repeats in the fragile-X gene, FMR1. This allele size is the smallest undergoing expansion to a full mutation (greater than 200 repeats) in one transmission. This expansion to a full mutation with greater than 200 repeats occurs only on transmission through females. Fragile X is a common cause of inherited mental retardation and is caused by an expansion of a CGG repeat within the gene. |