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微信小章| 产品名称： | C127I |
|---|---|
| 商品货号： | TS212542 |
| Organism： | Mus musculus, mouse |
| Tissue： | mammary gland |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Strain： | RIII |
| Applications： | Useful for visualization of sarcoma virus-induced foci and for an in vitro quantitative assay for bovine papilloma virus. Host for transfection with plasmids containing bovine papilloma virus DNA. C127I cells are suitable hosts for transformation with bovine papilloma virus DNA plasmids (ATCC 37110, ATCC 37111, ATCC 37112, ATCC 39475) |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Non-transformed clonal line derived from a mammary tumor of an RIII mouse. |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose; 90%; fetal bovine serum, 10% Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (Ref |
| Subculturing： | Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature (or 37°C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:15 is recommended
Medium Renewal: 2 to 3 times per week |
| Name of Depositor： | PM Howley |
| Deposited As： | Mus musculus |
| References： | Sarver N, et al. Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector. Mol. Cell. Biol. 1: 486-496, 1981. PubMed: 6100967 Lowy DR, et al. Helper-independent tranformation by unintegrated Harvey sarcoma virus DNA. J. Virol. 26: 291-298, 1978. PubMed: 26810 Dvoretzky I, et al. A quantitative in Vitro focus assay for bovine papilloma virus. Virology 103: 369-375, 1980. PubMed: 6247821 Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776 |
Berthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc. Natl. Acad. Sci. USA 83: 2496–2500, 1986. PubMed: 3458212