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| 产品名称： | BW5147.G.1.4.OUAR.1 |
|---|---|
| 商品货号： | TS212549 |
| Organism： | Mus musculus, mouse |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | AKR/J |
| Applications： | They can be used to create T cell hybridomas. Tested and found negative for ectromelia virus (mousepox). |
| Antigen Expression： | H-2k |
| Genes Expressed： | H-2k |
| Comments： | The cells are ouabain resistant. They can be used to create T cell hybridomas. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; horse serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 X 10 exp4 viable cells/ml. Subculture every 3 to 4 days. |
| Name of Depositor： | R Hyman |
| Deposited As： | Mus musculus |
| References： | Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. PubMed: 4544550 |