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微信小章| 产品名称： | BW5147.G.1.4 |
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| 商品货号： | TS212550 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | AKR/J |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from BW5147.3 (ATCC TIB-47). |
| Antigen Expression： | H-2k |
| Comments： | The cells are resistant to 0.1 mM 6-thioguanine. Unlike the parental line, these cells are sensitive to HAT. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: 5% CO2 |
| Name of Depositor： | R Hyman |
| Deposited As： | Mus musculus |
| References： | Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Kohler G, et al. Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7: 758-761, 1977. PubMed: 563329 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Goldsby RA, et al. Hybrid cell lines with T-cell characteristics. Nature 267: 707-708, 1977. PubMed: 301614 Hammerling GJ. T lymphocyte tissue culture lines produced by cell hybridization. Eur. J. Immunol. 7: 743-746, 1977. PubMed: 304002 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |