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微信小章| 产品名称： | BW5147.3(Thy-1-e).10 |
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| 商品货号： | TS212551 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | AKR/J |
| Applications： | This line is a Class E Thy-1 negative (Thy-1-) mutant derived from BW5147.3 (ATCC TIB-47). The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins. The cells are deficient in dolichol-P-mannose synthetase. The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain. |
| Derivation： | This line is a Class E Thy-1 negative (Thy-1-) mutant derived from BW5147.3 (ATCC TIB-47). |
| Comments： | This line is a Class E Thy-1 negative (Thy-1-) mutant derived from BW5147.3 (ATCC TIB-47). The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor. The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins. The cells are deficient in dolichol-P-mannose synthetase. The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; heat-inactivated horse serum, 10%
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor： | R Hyman |
| Deposited As： | Mus musculus |
| References： | Trowbridge IS, et al. The synthesis and properties of T25 blycoprotein in Thy-1-negative mutant lymphoma cells. Cell 14: 21-32, 1978. PubMed: 667934 Conzelmann A, et al. Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells. EMBO J. 5: 3291-3296, 1986. PubMed: 2880714 Chapman A, et al. The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose. J. Biol. Chem. 255: 4441-4446, 1980. PubMed: 7372584 |