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微信小章| 产品名称： | C 211 |
|---|---|
| 商品货号： | TS212553 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Cri du Chat syndrome |
| Age： | 11 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen storage temperature |
| Karyotype： | model number = 46; range = 39 to 95. Karyotype is that of a normal human diploid male with one 4 or 5 chromosome with a deleted short arm in all cells. The four cells with 47 chromosomes all had an extra G group chromosome. |
| Clinical Data： | male
Caucasian
11 years old |
| Comments： | The line has a life expectancy of approximately 21 passages. |
| Complete Growth Medium： | McCoyx92s 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 80%; fetal bovine serum, 20%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; CO2, 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12,13 D16S539: 9,11 D5S818: 9,12 D7S820: 10,11 THO1: 9.3 TPOX: 8 vWA: 16 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | WR Breg |
| Deposited As： | Homo sapiens |
| Passage History： | The line has a life expectancy of at least 30 passages. |
| References： | Miller OJ, et al. Alternative DNA replication patterns associated with long arm length of chromosomes 4 and 5 in the cri du chat syndrome. Cytogenetics 5: 137-151, 1966. PubMed: 5921496 Warburton D, et al. Distinction between chromosome 4 and chromosome 5 by replication pattern and length of long and short arms. Am. J. Hum. Genet. 19: 399-415, 1967. PubMed: 6026933 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |