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| 产品名称： | BT-549 |
|---|---|
| 商品货号： | TS212560 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | ductal carcinoma |
| Age： | 72 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 78; range = 73 to 80. The cell line is aneuploid human female, with chromosome counts in the hypertriploid range. The X chromosomes are all abnormal. Normal chromosomes N10 and N13 are clearly under-represented, with chromosomes N2, N12, and N17 also tending to be. Chromosome N8 is over-represented with respect to the copy number of other normal chromosomes, and chromosomes N5 and N18 also usually have more copies than most chromosomes. Four marker chromosomes are found: iso(13q), 10q+, del(X)(q22:), del(11)(p11:). |
| Images： |  |
| Derivation： | The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes. |
| Clinical Data： | 72 years Caucasian female |
| Comments： | The established population is polymorphic with epithelial like components and multinucleated giant cells. A mucin-like material was secreted into the medium. |
| Complete Growth Medium： | The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001). To make the complete growth medium, add the following components to the base medium: 0.023 U/ml insulin; fetal bovine serum (FBS; ATCC 30-2020) to a final concentration of 10%. |
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin : X CSF1PO: 10 , 12 D13S317: 11 D16S539 : 8 D5S818: 11 D7S820: 9, 10 TPOX: 8 THO1: 9.3 vWA : 15 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 2 PGM3, 1 |
| Name of Depositor： | W Coutinho, EY Lasfargues |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1978 |
| References： | Katayose Y, et al. Promoting apoptosis: a novel activity associated with the Cyclin-dependent kinase inhibitor p27. Cancer Res. 57: 5441-5445, 1997. PubMed: 9407946 Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 |