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| 产品名称： | BT-483 |
|---|---|
| 商品货号： | TS212562 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | ductal carcinoma |
| Age： | 23 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 72; range = 46 to 130 |
| Images： |  |
| Derivation： | The BT-483 line was isolated by E.Y. Lasfargues and W.G. Coutinho from a papillary invasive ductal tumor obtained from a 23 year old, normal, menstruating, parous female patient with family history of breast cancer. |
| Clinical Data： | 23 years Caucasian female |
| Tumorigenic： | Yes |
| Effects： | Yes, in Amsterdam/IMR rats Yes, in nude mice Yes, in soft agar |
| Comments： | The cells were highly pleomorphic with prominent nuclei and nucleoli PubMed ID: 212572. Epithelial characteristics including desmosomes, microvilli, tight and gap junctions were observed by transmission electron microscopy PubMed ID: 212572. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37°C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 9,12 D13S317: 8,12 D16S539: 11,12 D5S818: 11 D7S820: 9,10 THO1: 5,9.3 TPOX: 8,11 vWA: 17 |
| Isoenzymes： | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 2 PGM3, 2 |
| Name of Depositor： | EY Lasfargues |
| Deposited As： | Homo sapiens |
| References： | Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572 Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 |