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| 产品名称： | BVD2-23B6.4 |
|---|---|
| 商品货号： | TS212566 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain:xa0 Lewis (B cell); BALB/c (myeloma) |
| Applications： | The antibody binds to and blocks the activity of human GM-CSF. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Animals were immunized with purified human GM-CSF. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human granulocyte - macrophage colony stimulating factor (GM-CSF) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human granulocyte - macrophage colony stimulating factor (GM-CSF) |
| Comments： | Animals were immunized with purified human GM-CSF. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody binds to and blocks the activity of human GM-CSF. |
| Complete Growth Medium： | RPMI 1640 medium with 1 mM L-glutamine and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor： | Schering Corp. |
| Deposited As： | rat (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Abrams JS, VanDyke RE. Immunochemical assay for human granulocyte-macrophage colony stimulating factor. US Patent 5,070,013 dated Dec 3 1991 |