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| 产品名称： | BW5147(T200-a)5.2 |
|---|---|
| 商品货号： | TS212567 |
| Organism： | Mus musculus, mouse |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | AKR/J |
| Applications： | They are also negative for the CD45, T200, and Lea antigens. The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line is a derivative of BW5147.G.1.4.OUAR.1 (ATCC CRL-1588). |
| Comments： | This line is a derivative of BW5147.G.1.4.OUAR.1 (ATCC CRL-1588). The cells do not synthesize detectable amounts of the CD45 glycoprotein and do not express this molecule on the cell surface. They are also negative for the CD45, T200, and Lea antigens. The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
|
| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 to 8 X 10(4) viable cells/ml. Maintain cell density between 5 X 10(4) and 1 X 10(6) viable cells/ml. Do not exceed 5 X 10(5) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | R Hyman |
| Deposited As： | Mus musculus |
| References： | Hyman R, Trowbridge I. Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants. Immunogenetics 12: 511-523, 1981. PubMed: 7194313 |