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微信小章| 产品名称： | BLO-11 |
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| 商品货号： | TS212574 |
| Organism： | Mus musculus, mouse |
| Tissue： | skeletal muscle |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lysyl oxidase deficiency |
| Age： | 30 days |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was derived from a mouse carrying the blotchy mutation. |
| Antigen Expression： | H-2b |
| Comments： | The cells are deficient in lysyl oxidase. ATCC CCL-197 is an age, sex and strain matched control for this line. Tested and found negative for ectromelia virus (mousepox).
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:5 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley - Liss, N.Y., 2005. |
| Cryopreservation： | Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | RC Starcher, J Madaras |
| Deposited As： | Mus musculus |
| References： | Starcher BC, et al. Lysyl oxidase deficiency in lung and fibroblasts from mice with hereditary emphysema. Biochem. Biophys. Res. Commun. 78: 706-712, 1977. PubMed: 20889 Starcher B, et al. Abnormal cellular copper metabolism in the blotchy mouse. J. Nutr. 108: 1229-1233, 1978. PubMed: 27591 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |