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微信小章| 产品名称： | BRL 3A |
|---|---|
| 商品货号： | TS212575 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | liver |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Buffalo |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | BRL 3A was derived from buffalo rat liver cells |
| Genes Expressed： | Somatomedin like multiplication stimulating activity (MSA) |
| Cellular Products： | somatomedin like multiplication stimulating activity (MSA) |
| Comments： | The serum-free conditioned medium from this cell line is a source of MSA. MSA is a family of polypeptides that can partially satisfy the serum requirement of chick embryo fibroblasts. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% This medium is formulated for use with a 5% CO2 in air atmosphere. ATCC tested fetal bovine serum is available as ATCC® Catalog No. 30-2020 (500 mL) and ATCC® Catalog No. 30-2021 (100 mL).
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium supplemented with 10% fetal bovine serum and 5% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C   Nissley SP, et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146 |
| Name of Depositor： | SP Nissley, MM Rechler |
| Deposited As： | rat |
| References： | Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097 Dulak NC, Temin HM. A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153-170, 1973. PubMed: 4735141 Dulak NC, Shing YW. Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. J. Cell. Physiol. 90: 127-130, 1977. PubMed: 833209 Schalch DS, et al. Nonsuppressible insulin-like activity (NSILA). I. Development of a new sensitive competitive protein-binding assay for determination of serum levels. J. Clin. Endocrinol. Metab. 46: 664-671, 1978. PubMed: 755052 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Nissley SP, et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146 |
| Cross References： | Nucleotide (GenBank) : U14746 Rattus norvegicus VHL protein (VHL) mRNA, complete cds. |