微信小陈
微信小章| 产品名称： | BS-C-1 |
|---|---|
| 商品货号： | TS212577 |
| Organism： | Cercopithecus aethiops |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Applications： | This cell line is a suitable transfection host and may be used for the detection of viruses. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Chromosome Frequency Distribution 100 Cells: 2n = 60. One large submetacentric marker chromosome. Most of the cells contained a chromosome with a secondary constriction and many of the cells contained dicentric chromosomes. |
| Genes Expressed： | keratin |
| Cellular Products： | keratin |
| Virus Susceptibility： | Simian virus 40
,
Simian virus 40 Human poliovirus 1 Vesicular stomatitis, Orsay (Indiana) Vesicular stomatitis, Glasgow (Indiana) |
| Comments： | The cells are positive for keratin by immunoperoxidase staining.
African green monkey kidney cell line suitable for transfection by SV40 vectors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | HE Hopps |
| Deposited As： | Cercopithecus aethiops |
| References： | Hopps, H. E., et al. Biologic characteristics of the continuous kidney cell line derived from the African green monkey. J. Immunol. 91: 416-424, 1963. PubMed: 14071033 American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256 Jelachich ML, Lipton HL. Theilers murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327 Schmidt NJ, et al. The sensitivity of grivet monkey kidney cell line BS-C-1 for propagation and isolation of certain human viruses. Am. J. Public Health 54: 1522-1530, 1964. |