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微信小章| 产品名称： | BeWo |
|---|---|
| 商品货号： | TS212583 |
| Organism： | Homo sapiens, human |
| Tissue： | placenta |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | choriocarcinoma |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 86; range = 71 to 178. Stemline number is hypotetraploid. Karyotype relatively stable within stemline number. |
| Images： |  |
| Clinical Data： | male |
| Genes Expressed： | hormones; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol. The cells are positive for keratin by immunoperoxidase staining. |
| Cellular Products： | hormones; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol; keratin |
| Virus Susceptibility： | Human poliovirus 3
Vesicular stomatitis virus |
| Comments： | The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 3 to 4 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9,11 D16S539: 13,14 D5S818: 10,11 D7S820: 10,12 THO1: 9,9.3 TPOX: 8 vWA: 16 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | RA Pattillo |
| Deposited As： | Homo sapiens |
| References： | Pattillo RA, et al. Human hormone production in vitro. Science 159: 1467-1469, 1968. PubMed: 5753554 Pattillo RA, et al. Control mechanisms for gonadotrophic hormone production in vitro. In Vitro 6: 205-214, 1970. PubMed: 5535575 Pattillo RA, et al. Estrogen production by trophoblastic tumors in tissue culture. J. Clin. Endocrinol. Metab. 34: 59-61, 1972. PubMed: 4332667 Pattillo RA, Gey GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 28: 1231-1236, 1968. PubMed: 4299001 Hertz R. Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc. Soc. Exp. Biol. Med. 102: 77-81, 1959. PubMed: 14401422 Pattillo RA, et al. The hormone-synthesizing trophoblastic cell in vitro: a model for cancer research and placental hormone synthesis. Ann. N.Y. Acad. Sci. 172: 288-298, 1971. PubMed: 5289994 Schar BK, et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. PubMed: 9288797 Heckert LL, et al. The cAMP response elements of the alpha subunit gene bind similar proteins in trophoblasts and gonadotropes but have distinct functional sequence requirements. J. Biol. Chem. 49: 31650-31656, 1996. PubMed: 8940185 |