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微信小章| 产品名称： | BL3.1 |
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| 商品货号： | TS212585 |
| Organism： | Bos taurus, cow |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphosarcoma |
| Age： | 3 month old |
| Gender： | male |
| Strain： | Hereford |
| Applications： | CRL-2306 can be used in BLV and retroviral studies and as a target for NK assays. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3. The BL-3 cell line was isolated from a 3-month-old male Hereford calf. BL-3 cells were exposed to gamma-irradiation and surviving cells were immunoselected for MHC class I antigen loss. |
| Genes Expressed： | bovine leukemia virus (BLV) |
| Cellular Products： | bovine leukemia virus (BLV) |
| Comments： | BL3.1 cells are a major histocompatibility complex (MHC) transcriptional loss variant. The cells exhibit no expression of MHC class I and high expression of MHC class II. Few cell lines express only MHC class II. The cell line is positive for bovine leukemia virus (BLV) and actively produces BLV.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | JS Harms |
| Year of Origin： | 1990 |
| References： | Harms JS, Splitter GA. Impairment of MHC class I transcription in a mutant bovine B cell line. Immunogenetics 35: 1-8, 1992. PubMed: 1345904 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |