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产品简介
购买须知
| 产品名称： | BLK SV HD.2 A.5R.1 A.3R.1 |
|---|---|
| 商品货号： | TS212586 |
| Organism： | Mus musculus, mouse |
| Cell Type： | fibroblastSV40 transformed |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | embryo |
| Strain： | C57BL/6 |
| Applications： | This line was derived from BLK CL.4 (ATCC TIB-81) by transformation with SV40. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | This line was derived from BLK CL.4 (ATCC TIB-81) by transformation with SV40. |
| Tumorigenic： | Yes |
| Effects： | Yes, in ATxFL mice but not in normal C57BL/6 mice |
| Comments： | This line was derived from BLK CL.4 (ATCC TIB-81) by transformation with SV40. The cells will grow in 0.3% agarose. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove old medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Name of Depositor： | P Patek, J Collins, M Cohn |
| Deposited As： | Mus musculus |
| References： | Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934 |