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| 产品名称： | BE2 |
|---|---|
| 商品货号： | TS212591 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against glycosphingolipid having type II chain H structure |
| Cellular Products： | immunoglobulin; monoclonal antibody; against glycosphingolipid having type II chain H structure |
| Comments： | Animals were immunized with naked Salmonella minnesota to which had been adsorbed a mixture of neutral glycolipids from human type O erythrocytes. Spleen cells were fused with NS-1 myeloma cells. At 37C, this antibody agglutinates type O and A2 but not type B or A1 erythrocytes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | WW Young |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Young WW Jr., et al. Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-n-glycosyl type II chain. J. Biol. Chem. 256: 10967-10972, 1981. PubMed: 7287743 |