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| 产品名称： | BE(2)-M17 |
|---|---|
| 商品货号： | TS212592 |
| Organism： | Homo sapiens, human |
| Tissue： | brain; derived from metastatic site: bone marrow |
| Cell Type： | neuroblast |
| Product Format： | frozen |
| Morphology： | neuroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Age： | 2 years |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Derivation： | BE(2)-M17 is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from a 2 year old male with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy. |
| Clinical Data： | Patient had repeated courses of chemotherapy and radiotherapy. male |
| Comments： | BE(2)-M17 cells have a reported saturation density of greater than 1 x 106 cells/cm2
The cells grow as multilayering, neuroblastic cells with occasionally long neuritic processes.
As cultures age, cells will aggregate and float. |
| Complete Growth Medium： | The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Remove medium carefully. Add 1-2 mL trypsin (0.25%) - EDTA (0.53mM) solution and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach. Add fresh culture medium, triturate cells and dispense into new culture flasks. Subcultivation ratio: 1:20 to 1:50 at 1 to 2 week intervals is recommended Medium Renewal: Every 4 to 7 days. pH should be less than or equal to 7.3. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 9,10 THO1: 6 TPOX: 8,11 vWA: 18 |
| Population Doubling Time： | 20 to 24 hrs |
| Name of Depositor： | JL Biedler |
| Deposited As： | Homo sapiens |