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微信小章| 产品名称： | BEND |
|---|---|
| 商品货号： | TS212593 |
| Organism： | Bos taurus, cow |
| Tissue： | uterus, endometrium |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain bovine viral diarrhea virus (BVDV)
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult; day 14 of estrous cycle |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The BEND cell line was derived from the uterine endometrium of a normal female cow on day 14 of the estrous cycle in 1997 in Laramie Wyoming, United States. |
| Cellular Products： | ubiquitin cross-reactive protein (UCRP) and alpha chemokines in response to interferon |
| Comments： | The cells produce the pregnancy associated protein called bovine ubiquitin cross-reactive protein (UCRP) and alpha chemokines in response to the cytokine interferon-tau.
BEND was submitted to the American Type Culture Collection in January, 1998 at passage 6. Tests for bovine viral diarrhea virus (BVDV) at the ATCC have indicated that BEND cells are positive for BVDV by immunofluorescence. |
| Complete Growth Medium： | 1:1 mixture of Hams F12 and Eagles Minimal Essential medium with Earles BSS (D-valine modification) with 1.5 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.034 g/L D-valine, 10% heat-inactivated fetal bovine serum and 10% heat-inactivated horse serum
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | TR Hansen, KJ Austin, GA Johnson |
| Deposited As： | Bos taurus |
| Passage History： | BEND was submitted to the American Type Culture Collection in January, 1998 at passage 6. |
| Year of Origin： | 1997 |
| References： | Staggs KL, et al. Complex induction of bovine uterine proteins by interferon-tau. Biol. Reprod. 59: 293-297, 1998. PubMed: 9687298 Hansen TR, et al. Transient ubiquitin cross-reactive protein gene expression in the bovine endometrium. Endocrinology 138: 5079-5082, 1997. PubMed: 9348245 Austin KJ, et al. Pregnancy-specific protein B induces release of an alpha chemokine in bovine endometrium. Endocrinology 140: 542-545, 1999. PubMed: 9886868 Johnson GA, et al. Endometrial ISG17 mRNA and a related mRNA are induced by interferon-tau and localized to glandular epithelial and stromal cells from pregnant cows. Endocrine 10: 243-252, 1999. PubMed: 10484288 Perry DJ, et al. Cloning of interferon-stimulated gene 17: the promoter and nuclear proteins that regulate transcription. Mol. Endocrinol. 13: 1197-1206, 1999. PubMed: 10406469 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |