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微信小章| 产品名称： | Beta-TC-6 |
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| 商品货号： | TS212597 |
| Organism： | Mus musculus, transgenic for SV40 large T antigen, mouse, transgenic for SV40 large T antigen |
| Tissue： | pancreas |
| Cell Type： | beta cell |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain SV40 viral DNA Sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | insulinoma |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | The cell line was derived from a pancreatic tumor (insulinoma) arising in a transgenic mouse. |
| Genes Expressed： | insulin, glucagon, and somatostatin |
| Cellular Products： | insulin, glucagon and somatostatin |
| Comments： | They secrete insulin in response to glucose. The mouse carried the pseudogene construct composed of the SV40 early region controlled by the rat insulin II gene promotor. The cells contain abundant insulin and small amounts of glucagon and somatostatin. They secrete insulin in response to glucose. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | CytoTherapeutics, Inc. |
| Deposited As： | mouse, transgenic for SV40 large T antigen |
| U.S. Patent Number： | |
| References： | Laurance ME, et al. Glucose responsive insulin secreting beta-cell lines and method for producing same. US Patent 5,773,255 dated Jun 30 1998 Poitout V, et al. Morphological and functional characterization of beta TC-6 cells--an insulin-secreting cell line derived from transgenic mice. Diabetes 44: 306-313, 1995. PubMed: 7533732 Poitout V, et al. Insulin-secreting cell lines: classification, characteristics and potential applications. Diabetes Metab. 22: 7-14, 1996. PubMed: 8697299 |