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| 产品名称： | bcd mab23 |
|---|---|
| 商品货号： | TS212603 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the maternally encoded bcd protein (a morphogen in the Drosophila embryo). |
| Derivation： | Animals were immunized with purified LacZ - bcd fusion protein and boosted with TrpE - bcd fusion protein. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the bicoid (bcd) protein of Drosophila melanogaster |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the bicoid (bcd) protein of Drosophila melanogaster |
| Comments： | Animals were immunized with purified LacZ - bcd fusion protein and boosted with TrpE - bcd fusion protein. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody reacts with the maternally encoded bcd protein (a morphogen in the Drosophila embryo). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Name of Depositor： | W Driever |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Driever W, Nusslein-Volhard C. A gradient of bicoid protein in Drosophila embryos. Cell 54: 83-93, 1988. PubMed: 3383244 Driever W, Nusslein-Volhard C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. Cell 54: 95-104, 1988. PubMed: 3383245 |