微信小陈
微信小章| 产品名称： | BCL1 clone 5B1b |
|---|---|
| 商品货号： | TS212604 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | leukemia; lymphoma |
| Gender： | female |
| Strain： | BALB/c |
| Applications： | The cells can be activated by LPS to produce IgM and can be used as a target for B cell growth factor (BCGF) assays.
|
| Images： |  |
| Clinical Data： | female |
| Genes Expressed： | immunoglobulin (surface, sIg +); monoclonal antibody |
| Cellular Products： | immunoglobulin (surface, sIg +); monoclonal antibody |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine and 0.05 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Subcultures are prepared by scraping. Dislodge cells in the spent medium, aspirate, add fresh medium and dispense into new flasks. |
| Isotype： | IgD; IgM; lambda light chain |
| Name of Depositor： | S Strober |
| Deposited As： | Mus musculus |
| References： | Gronowicz ES, et al. An in vitro line of the B cell tumor BCL1 can be activated by LPS to secrete IgM1. J. Immunol. 125: 976-980, 1980. PubMed: 6157730 Thoman ML, Weigle WO. Identification and partial purification of a B cell growth-promoting material utilizing the BCL1 cell line. J. Immunol. 130: 233-236, 1983. PubMed: 6600179 Strober S, et al. Immunobiology of a spontaneous murine B cell leukemia (BCL1). Immunol. Rev. 48: 169-195, 1979. PubMed: 121100 Slavin S, Strober S. Spontaneous murine B-cell leukaemia. Nature 272: 624-626, 1978. PubMed: 306069 |