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| 产品名称： | BE(2)-C |
|---|---|
| 商品货号： | TS212605 |
| Organism： | Homo sapiens, human |
| Tissue： | brain; derived from metastatic site: bone marrow |
| Cell Type： | neuroblast, Neuroblastoma |
| Product Format： | frozen |
| Morphology： | neuroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Age： | 2 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | BE(2)-C is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy. |
| Clinical Data： | BE(2)-C is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from a child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy. male |
| Comments： | BE(2)-C cells have a reported saturation density of greater than 5 X 105 cells/cm2.
The cells grow as clusters of flattened neuroblastic cells with occasional fine cell processes (neurites).
Unlike the parent line, they generally do not detach and float. |
| Complete Growth Medium： | The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:6 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 9,11 D5S818: 12 D7S820: 9,10 THO1: 6 TPOX: 11 vWA: 18 |
| Population Doubling Time： | 18 hrs |
| Name of Depositor： | JL Biedler |
| Deposited As： | Homo sapiens |
| Year of Origin： | November, 1972 |