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微信小章| 产品名称： | BCP-1 |
|---|---|
| 商品货号： | TS212606 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 CELLS CONTAIN HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | body cavity based lymphoma |
| Age： | 92 years |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | BCP-1 is a clonal lymphoma cell line isolated in 1995 by serial dilution of peripheral blood mononuclear cells from an HIV seronegative patient with body cavity based lymphoma (BCBL). |
| Comments： | The BCP-1 cell line is positive for the Kaposis sarcoma associated herpesvirus (KSHV, provisionally designated Human Herpes Virus-8 or HHV-8) genome at 150 copies/cell. BCP-1 is negative for Epstein-Barr virus (EBV), HHV-6 and HHV-7. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Interval: Maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium supplemented with 7.5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | SJ Gao, PS Moore, Y Chang |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1995 |
| References： | Gao SJ, et al. KSHV antibodies among Americans, Italians and Ugandans with and without Kaposis sarcoma. Nat. Med. 2: 925-928, 1996. PubMed: 8705864 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |