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| 产品名称： | BBM |
|---|---|
| 商品货号： | TS212613 |
| Organism： | Homo sapiens, human |
| Tissue： | lung, bronchus |
| Cell Type： | epithelial virus transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Carcinogen |
| Applications： | Tranformants were selected in medium containing G418. The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc. This line was derived from BEAS-2B cells (see ATCC CRL-9609) by transfection with the B-myc/pSV2neo plasmid (constructed by ligating a BamH1/EcoR1 fragment of the c-myc gene from CA46 cells to a BamH1/EcoR1 fragment of the pSV2neo plasmid). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | This line was derived from BEAS-2B cells (see ATCC CRL-9609) by transfection with the B-myc/pSV2neo plasmid (constructed by ligating a BamH1/EcoR1 fragment of the c-myc gene from CA46 cells to a BamH1/EcoR1 fragment of the pSV2neo plasmid). Tranformants were selected in medium containing G418. The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc. The cells are reported to stain positively for keratins and SV40 T antigen. |
| Tumorigenic： | No, |
| Effects： | Yes, did form colonies in semisolid medium. No, the cells were not tumorigenic in immunosuppressed mice, |
| Comments： | This line was derived from BEAS-2B cells (see ATCC CRL-9609) by transfection with the B-myc/pSV2neo plasmid (constructed by ligating a BamH1/EcoR1 fragment of the c-myc gene from CA46 cells to a BamH1/EcoR1 fragment of the pSV2neo plasmid). Tranformants were selected in medium containing G418. The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc. The cells are reported to stain positively for keratins and SV40 T antigen. |
| Complete Growth Medium： | The base medium for this cell line (BEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: BEGM, Kit Catalog No. CC-3170. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with the BEGM kit.
Note: Do not filter complete medium.
|
| Subculturing： | Protocol:
Subcultivation Ratio: Inoculate new flasks at 1500 to 3000 cells per sq. cm. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze medium: Leibovitzs L-15 medium with 2 mM L-glutamine supplemented with 10 mM HEPES, 1% PVP, 10% fetal bovine serum and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: XY CSF1PO: 9, 12 D13S317: 13 D16S539: 12 D5S818: 12, 13 D7S820: 10, 13 THO1: 7, 9.3 TPOX: 6, 11 vWA: 17, 18 |
| Name of Depositor： | The United States of America |
| U.S. Patent Number： | |
| References： | Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989 Lechner JF, LaVeck MA. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Culture Methods 9: 43-48, 1985. |