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| 产品名称： | BB7.5 |
|---|---|
| 商品货号： | TS212614 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with a combinatorial determinant of HLA A,B,C. The antibody cross-reacts with cells of various primates. The cells do poorly if hypoxanthine and thymidine are omitted from the medium tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against HLA A, B, C |
| Cellular Products： | immunoglobulin; monoclonal antibody; against HLA A, B, C |
| Comments： | Animals were immunized with papain solubilized HLA A2,B7. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with a combinatorial determinant of HLA A,B,C. The antibody cross-reacts with cells of various primates. The cells do poorly if hypoxanthine and thymidine are omitted from the medium tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640 with 10(-4) M hypoxanthine, 1.6 x 10(-5) M thymidine, 90%; fetal bovine serum, 10%. The cells do poorly if hypoxanthine and thymidine are omitted. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | P Parham |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Brodsky FM, Parham P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J. Immunol. 128: 129-135, 1982. PubMed: 6172474 Brodsky FM, et al. Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47: 3-61, 1979. PubMed: 95015 |