微信小陈
微信小章
产品简介
购买须知
| 产品名称： | BALB/B 0.75BAE A.1R.1 HD A.8 |
|---|---|
| 商品货号： | TS212622 |
| Organism： | Mus musculus, mouse |
| Cell Type： | fibroblastchemically transformed |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | embryo |
| Strain： | BALB.B |
| Applications： | This line was derived from the BALB/B CL.6 normal embryonic fibroblast cell line by transformation with benzanthracene epoxide. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from the BALB/B CL.6 normal embryonic fibroblast cell line by transformation with benzanthracene epoxide. |
| Tumorigenic： | No |
| Effects： | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments： | This line was derived from the BALB/B CL.6 normal embryonic fibroblast cell line by transformation with benzanthracene epoxide. The cells will grow in 0.3% agarose. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | P Patek, J Collins, M Cohn |
| Deposited As： | Mus musculus |
| References： | Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934 |