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| 产品名称： | BB |
|---|---|
| 商品货号： | TS212626 |
| Organism： | Ictalurus nebulosus, bullhead, brown |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 2 years |
| Virus Resistance： | herpes simplex; poliovirus 2; vaccinia |
| Comments： | BB cells will grow at 4 to 34C with an optimum of between 25 and 30C. The cells do not do well when cultured on a glass surface. |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2mM L-glutamine and Hanks BSS with 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 1 to 2 times per week Remove medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove trypsin and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks. |
| Culture Conditions： | Temperature: 23.0°C |
| Name of Depositor： | RG Malsberger |
| Deposited As： | Ictalurus nebulosus |
| References： | Wolf K, Quimby MC. Fish cell and tissue culture In: Wolf K, Quimby MC. Fish physiology, 3 New York Academic Press pp. 253-301, 1969 |