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微信小章| 产品名称： | B-3 |
|---|---|
| 商品货号： | TS212628 |
| Organism： | Homo sapiens, human |
| Tissue： | eye; lens |
| Cell Type： | transformed with an adenovirus 12-SV40 virus hybrid (Ad12-SV40) |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Adenovirus-SV-40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | infant less than 1 year |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | The cell line was derived from a human lens obtained within 24 hrs from 5-12 month old patient who underwent treatment for retinopathy of prematurity.xa0At 60% confluence, cells at passage 3 were infected with Ad12-SV40. |
| Clinical Data： | The cell line was derived from a human lens obtained within 24 hrs from 5-12 month old patient who underwent treatment for retinopathy of prematurity. |
| Antigen Expression： | SV40 T antigen Ref   Andley UP, et al. Propagation and immortalization of human lens epithelial cells in culture. Invest. Ophthalmol. Vis. Sci. 35: 3094-3102, 1994. PubMed: 8206728 |
| Genes Expressed： | beta and gamma crystallin
(Andley UP, Fleming TP. Immortalized epithelial cell lines. US Patent 5,643,782 dated Jul 1 1997)
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| Cellular Products： | beta and gamma crystallin
(Andley UP, Fleming TP. Immortalized epithelial cell lines. US Patent 5,643,782 dated Jul 1 1997) |
| Comments： | The cells synthesize beta and gamma crystallins as monitored by immunoblot assay.
The cells can be maintained in culture for over 76 population doublings with no decrease in proliferative capability. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | These cells are very sensitive to trypsin-EDTA.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | T Fleming, U Andley |
| U.S. Patent Number： | |
| References： | Andley UP, Fleming TP. Immortalized epithelial cell lines. US Patent 5,643,782 dated Jul 1 1997 Andley UP, et al. Propagation and immortalization of human lens epithelial cells in culture. Invest. Ophthalmol. Vis. Sci. 35: 3094-3102, 1994. PubMed: 8206728 Fleming TP, et al. Expression of growth control and differentiation genes in human lens epithelial cells with extended life span. Invest. Ophthalmol. Vis. Sci. 39: 1387-1398, 1998. PubMed: 9660487 |